Molecular Cloning of Equine Preprorelaxin cDNA

Molecular cloning of mouse placental lactogen cDNA.

We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage lambda gt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDa polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-I...

Molecular cloning of a canine metallothionein cDNA.

A canine metallothionein cDNA obtained from the liver of a cadmium-treated beagle was cloned and sequenced. Asn at position 4 conserved among all mammalian metallothionein-1 and metallothionein-2 is replaced by Asp in the canine metallothionein cDNA clone. Because the acidic amino acid doesn't exist at either position 10 or 11 in the deduced amino acid sequence, it is supposed that this cDNA is...

Molecular Cloning of Rat USF 2 cDNA of Splicing Variants

the entire sequence but encocled short peptides of an N-terminal portion of USF2FL. Overexpressien of USF2FL increased the transcription of the human high aMnity IgE receptor (FceRI) a chain gene through specific binding to the CAGCTG motif in the firstintron. On the other hand, oyerexpression of USF2Al or A2 reduced the transcription of the human FceRI a chain gene. Both USF2FL and USF2Al boun...

Molecular Cloning of an Alternative Human aE-Catenin cDNA

Molecular cloning and characterization of cDNA for human myeloperoxidase.

Partial amino acid sequence of human myeloperoxidase was determined, and a 41-base oligonucleotide containing deoxyinosines at four positions was chemically synthesized. By using the oligonucleotide as a probe, cDNA clones for human myeloperoxidase were isolated from a cDNA library constructed with mRNA from human promyelocytic leukemia HL-60 cells. One of the clones containing a 2.6-kilobase i...